Spectrometer

We have no spectrometer assembled yet. Actually we have just cutted the pieces by laser cut and collected the necessary diodes from old scanner devices. We intend to finish it as soon as possible.

Anúncios

Bioreactor

Our bioreactor is not ready yet, but our plan is to build it using algae and light  so they can survive well. We now have a partnership with some researchers of the University of São Paulo wich work with bioreactor and are also interested in building their oun devices with a lower cost.

The Centrifuge

A centrifuge has many applications inside the laboratory, although it´s role is always the same: to make use of the centrifuge force to separate or purify samples.
In the constriction fo our centrifuge we built it, printed the cage for the tubes and puted it all together with the rotor. However, we had a problem with the Arduino code while trying to make it work. After some days we had help from an experienced guy in the issue (Arduino) then it all went well in the end.

Centrifuge with view of the display
Centrifuge with view of the display   
Superior view of the centrifuge
Superior view of the centrifuge

Sterile Hood

A sterile hood is essential for keeping the purity of the environment and avoiding other microrganisms of getting into our experiment. The idea is to keep the air from outside, well… outside and vice versa.
During the construction of our sterile hood we had some problems because of a delay in the delivery of the materials so we couldn´t testit yet. But, the mechanisms seems to be working perfectly.

Superior view of the sterile hood
Superior view of the sterile hood

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Air system of the almost-ready sterile hood
Air system of the almost-ready sterile hood

Yeastogram, or the art of colored culture medium

The plans for this week are basically BE AN ARTIST! At least that is how I feel when preparing colored culture medium to performe the technique called Yeastogram. The idea is to grow yeast in a specific shape using a stencil and UV light. The stencil defines where the light can pass through and where it cannot, then the colonies grow just where the light couldn´t reach them, while the other died due to the huge amount of DNA damage caused by the full-of-energy rays. Yeah, I agree it´s AWESOME! The original suggestion was to use active coal to give the medium some color but I also used Methylene Blue and Safranin. I must say the results were quite satisfying, even the work is not co

Biohack Academy logo!
Biohack Academy logo!

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Cockroach Stencil! A future yeastogram
Cockroach Stencil! A future yeastogram
Garagem FabLab logo and cockroach stencils over the about-to-irradiate plates
Garagem FabLab logo and cockroach stencils over the about-to-irradiate plates
Now we have the biohack logo too
Now we have the biohack logo too
My hacked irirradiation chamber
My hacked irirradiation chamber
UV Light source
UV Light source
Sterile area around the flame
Sterile area around the flame
Methylene Blue culture medium :)
Methylene Blue culture medium 🙂

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Bottles
Bottles
Pressure cooker (yeah, it´s on the ground)
Pressure cooker (yeah, it´s on the ground)

Up to now, we couldn´t make the yeastogram to work but we keep trying. After some discussions and chating with some other people I suppose it might have gone wrong because of the differente sprectra of UV, even I knwe our lamp (we didn´t use the Led) was about 400nm. Also it was told me the distance between the lamp and the plates migh have been an important factor in the experiment. I am not sure yet if the stencil we used is allowing the passage of the light.

The Microscope!

As part of science development and expression of the curiosity caracteristic of the human being, the microscopic world has also been investigated for the last centuries. The main problem is the difficulty of one to obtain these equipments, mostly in Brazil, due to expensive taxes and hard access to this kind of information. In this context, Biohack Academy has provided us with a low cost model of microscope, easy to do by yourself and adaptable according to your needs and creativity.

Here are some pictures of my microscope and some samples I was able to magnify using it

Onion cells
Onion cells
Onion cells with visible nuclei
Onion cells with visible nuclei
Tilia stem showing different tissues of the plant stalk
Tilia stem showing different tissues of the plant stalk
Corn Root with visible nuclei
Corn Root with visible nuclei
Human fibroblasts, Crystal Violet
Human fibroblasts, Crystal Violet